3OUW

Structure of beta-catenin with Lef-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97922
Spacegroup name	I 4 2 2
Unit cell lengths	116.930, 116.930, 214.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.940 - 2.910
Rwork	0.203
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	beta-catenin arm domain from beta-catenin/phosphorylated Lef-1 structure
RMSD bond length	0.008
RMSD bond angle	1.191
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.102	0.396
Number of reflections	15960
<I/σ(I)>	13	2.1
Completeness [%]	96.5	82.1
Redundancy	4.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	well solution: 4.2% PEG 4000, 100 mM sodium citrate. protein solution: 100 uM beta-cat-arm, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K