3OUU
Crystal Structure of Biotin Carboxylase-beta-gamma-ATP Complex from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.830, 100.170, 148.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.915 - 2.252 |
R-factor | 0.1721 |
Rwork | 0.170 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | crystal structure of Biotin Carboxylase complexed with ADP |
RMSD bond length | 0.011 |
RMSD bond angle | 1.287 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 64184 | |
<I/σ(I)> | 8 | 2.6 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.16M Calcium acetate, 0.08M Sodium Cacodylate-HCl pH 6.5, 14.4% (w/v) PEG 8000, 20% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |