3OUT
Crystal structure of glutamate racemase from Francisella tularensis subsp. tularensis SCHU S4 in complex with D-glutamate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-13 |
| Detector | MAR CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 98.060, 98.060, 154.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.220 - 1.650 |
| R-factor | 0.16416 |
| Rwork | 0.163 |
| R-free | 0.19270 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.537 |
| Data scaling software | XSCALE |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.091 | 0.499 |
| Number of reflections | 172747 | |
| <I/σ(I)> | 14.71 | 3.16 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.6 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 25% PEG 3350, 0.1 M Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
