3OUR
Crystal structure of complex between EIIA and a novel pyruvate decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 322.866, 62.067, 126.362 |
Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 119307 | |
Completeness [%] | 98.5 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 295 | 20% PEG MME 550, 0.1M MES pH 6.5, 0.01M Zn-Acetate, microbatch, temperature 295K |