3OUL
Crystal Structure of toxoflavin-degrading enzyme in a substrate-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2398 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.377, 110.377, 56.787 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| Rwork | 0.195 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | structure from MAD data using a different mutant |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.275 |
| Number of reflections | 32823 | |
| Completeness [%] | 98.5 | 88.5 |
| Redundancy | 6.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M CAPS, pH 10.5, 0.2M LiSO4, 1.2M NaH2PO4/0.8M K2HPO4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
