3OUK
Crystal structure of Rv3910 from Mycobacterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115872 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.360, 122.360, 66.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.172 - 3.402 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.23450 |
| Structure solution method | SAD |
| Starting model (for MR) | phased from SeMet crystal |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | ELVES |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.172 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 7693 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 94.4 | 71.8 |
| Redundancy | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 3M NaCl, 0.1M Tris-HCl, pH 7.5-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | |
| 2 | VAPOR DIFFUSION, HANGING DROP | 291 | 60% tacsimate, 4% 1,1,1,3,3,3 hexafluoro-2-propanol , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
