3OU2
DhpI-SAH complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.519, 75.519, 70.657 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.19907 |
| Rwork | 0.198 |
| R-free | 0.22132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ou6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.353 | |
| Number of reflections | 36451 | |
| <I/σ(I)> | 38.1 | 3.9 |
| Completeness [%] | 99.4 | 95.2 |
| Redundancy | 8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 273 | 1.6 M Ammonium sulphate, 100 mM Tric-HCl pH=8.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
