3OTV
Crystal structure of the intracellular domain of Rv3910 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.037, 59.687, 145.063 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 40.814 - 3.094 |
| R-factor | 0.1924 |
| Rwork | 0.189 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ouk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (- Phaser) |
| Refinement software | ELVES |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.814 | 3.210 |
| High resolution limit [Å] | 3.094 | 3.100 |
| Rmerge | 0.631 | |
| Number of reflections | 15986 | |
| <I/σ(I)> | 9.9 | 1.73 |
| Completeness [%] | 87.4 | 70.8 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.7-0.8M succinate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
