3OTO

Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	402.089, 402.089, 173.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.842 - 3.690
R-factor	0.1757
Rwork	0.173
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqe
RMSD bond length	0.005
RMSD bond angle	0.759
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.760
High resolution limit [Å]	3.690	9.930	3.690
Rmerge	0.132	0.072	0.589
Number of reflections	148472
<I/σ(I)>	10.3		1.8
Completeness [%]	98.2	91.6	97.3
Redundancy	3.2	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	pH 6.5, hanging drop, temperature 277K