3OTB
Crystal structure of human tRNAHis guanylyltransferase (Thg1) - dGTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 104 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 140.912, 140.912, 81.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.950 |
Rwork | 0.254 |
R-free | 0.30140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.232 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.060 |
High resolution limit [Å] | 2.950 | 6.340 | 2.950 |
Rmerge | 0.086 | 0.042 | 0.957 |
Number of reflections | 17903 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 99.7 | 97.3 | 99.9 |
Redundancy | 14 | 13 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 298 | Na Formate, pH 7, hanging drop, temperature 298K |