3OSX

Crystal Structure of Apical Domain of Insecticidal GroEL from Xenorhapdus nematophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.548
Spacegroup name	P 21 21 21
Unit cell lengths	39.983, 61.882, 75.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.470 - 1.550
R-factor	0.2052
Rwork	0.148
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1la1
RMSD bond length	0.013
RMSD bond angle	1.235
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Auto-Rickshaw
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	24.470
High resolution limit [Å]	1.550
Number of reflections	27300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium Chloride, vapor diffusion, hanging drop, temperature 291K