3OSJ

X-Ray Structure of Phycobilisome LCM core-membrane linker polypeptide (fragment 254-400) from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR209C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-25
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	53.568, 79.607, 73.705
Unit cell angles	90.00, 95.50, 90.00

Refinement procedure

Resolution	41.290 - 2.300
R-factor	0.167
Rwork	0.162
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230
Rmerge	0.108
Number of reflections	58232
<I/σ(I)>	16.7
Completeness [%]	98.4
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: NaNo3 - 0.1M, MES - 0.1M, PEG 4000 - 20%, microbatch under oil, temperature 277K