3OS7
Crystal structure of a galactose mutarotase-like protein (CA_C0697) from CLOSTRIDIUM ACETOBUTYLICUM at 1.80 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-26 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 209.532, 41.553, 211.641 |
Unit cell angles | 90.00, 119.58, 90.00 |
Refinement procedure
Resolution | 29.553 - 1.800 |
R-factor | 0.1447 |
Rwork | 0.143 |
R-free | 0.18080 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.460 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.553 | 29.553 | 1.860 |
High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
Rmerge | 0.057 | 0.025 | 0.332 |
Number of reflections | 146955 | 27829 | 22516 |
<I/σ(I)> | 10.27 | 26.1 | 2 |
Completeness [%] | 93.5 | 95.8 | 83.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.2% polyethylene glycol 3350, 0.257M di-sodium tartrate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K | |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.2% polyethylene glycol 3350, 0.257M di-sodium tartrate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |