3OS2
PFV target capture complex (TCC) at 3.32 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 160.951, 160.951, 127.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.380 - 3.320 |
| R-factor | 0.22535 |
| Rwork | 0.224 |
| R-free | 0.25956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l2r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.040 | 3.500 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.090 | 0.990 |
| Number of reflections | 25361 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 34% PEG-400, 180mM Li2SO4, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
