3ORC
CRYSTAL STRUCTURE OF AN ENGINEERED CRO MONOMER BOUND NONSPECIFICALLY TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-02 |
| Detector | SDMS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.720, 60.660, 45.720 |
| Unit cell angles | 90.00, 112.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.224 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1orc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 19.550 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Refinement software | TNT (5EB) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.053 * |
| Total number of observations | 13041 * |
| Number of reflections | 2452 |
| Completeness [%] | 99.7 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | drop solution was mixed with an equal volume of precipitant * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | AMMONIUM ACETATE | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | ACETATE BUFFER | ||
| 4 | 1 | 2 | AMMONIUM ACETATE | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | ACETATE BUFFER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | DNA duplex | 13 (mg/ml) | |
| 5 | 1 | reservoir | ammonium acetate | 80-200 (mM) | precipitant |
| 6 | 1 | reservoir | PEG3350 | 29-33 (%) | precipitant |
| 7 | 1 | reservoir | acetate | 100 (mM) | precipitant |
