3OR5
Crystal structure of thiol:disulfide interchange protein, thioredoxin family protein from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.070, 50.070, 116.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.990 - 1.660 |
| R-factor | 0.17541 |
| Rwork | 0.174 |
| R-free | 0.19876 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.660 |
| Rmerge | 0.116 | 0.480 |
| Number of reflections | 18223 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 27.9 | 28.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M BIS-TRIS pH 6.5, 3.0 M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
