3OR1
Crystal structure of dissimilatory sulfite reductase I (DsrI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-08-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.721, 60.478, 132.915 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.760 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.071 | 0.381 |
| Number of reflections | 179998 | |
| <I/σ(I)> | 18.7 | 2.9 |
| Completeness [%] | 99.3 | |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 23%(W/V) PEG6000, 200MM SODIUM CHLORIDE, 100MM MES BUFFER, pH 5.5, VAPOR DIFFUSION, HANGING DROP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 23%(W/V) PEG6000, 200MM SODIUM CHLORIDE, 100MM MES BUFFER, pH 5.5, VAPOR DIFFUSION, HANGING DROP |
