3OQY
Semi-synthetic ribonuclease S: para-cyano-phenylalanine at position 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Collection date | 2004-09-16 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.906, 32.520, 68.932 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 41.052 - 1.494 |
| R-factor | 0.2028 |
| Rwork | 0.202 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rnv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.990 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.921 |
| High resolution limit [Å] | 1.494 |
| Number of reflections | 34995 |
| Completeness [%] | 95.8 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 279 | 25% PEG 4000, 0.08M ammonium acetate, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
