3OQU
Crystal structure of native abscisic acid receptor PYL9 with ABA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2010-07-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 112.024, 112.024, 40.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.510 - 2.680 |
| R-factor | 0.17881 |
| Rwork | 0.177 |
| R-free | 0.20416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3klx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.084 | 0.393 |
| Number of reflections | 15887 | |
| <I/σ(I)> | 16 | 2.31 |
| Completeness [%] | 1.0 | 95.2 |
| Redundancy | 6.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 30% peg 4000, 0.1M tris, 0.2M li2So4, pH 8.5, EVAPORATION, temperature 293K |
