3OQT

Crystal structure of Rv1498A protein from mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-20
Detector	Bruker Platinum 135
Spacegroup name	P 21 3
Unit cell lengths	143.946, 143.946, 143.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.880
R-factor	0.25382
Rwork	0.252
R-free	0.28285
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cc7
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.880	2.880
Number of reflections	22825
<I/σ(I)>	8.8
Completeness [%]	100.0	100
Redundancy	43.9	41.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8	295	2M NH4H2PO4SODIUM, 100 MILLIMOLAR TRIS (PH 8.5), temperature 295K