3OQL

Crystal structure of a TenA homolog (PSPTO1738) from Pseudomonas syringae pv. tomato str. DC3000 at 2.54 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97922,0.97898
Spacegroup name	P 41 2 2
Unit cell lengths	67.339, 67.339, 479.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 2.540
R-factor	0.1777
Rwork	0.176
R-free	0.20160
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.880		2.640
High resolution limit [Å]	2.540	5.460	2.550
Rmerge	0.074	0.035	0.822
Number of reflections	37971	7085	6488
<I/σ(I)>	11.66	28.9	1.6
Completeness [%]	99.3	99.1	94.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.21	293	0.84M sodium citrate, 0.1M Imidazole pH 8.21, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K