3OQE
Structure of OpdA mutant Y257F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.048, 109.048, 62.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.670 - 1.900 |
| R-factor | 0.17824 |
| Rwork | 0.176 |
| R-free | 0.22823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d2j |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.471 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.220 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | |
| Number of reflections | 32952 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 14.4% PEG 8000, 20% glycerol, 0.16M calcium acetate, 80mM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
