3OQC
Ubiquitin-fold modifier 1 Specific Protease, UfSP2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97947, 0.979613, 0.97177, 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.533, 56.041, 143.269 |
| Unit cell angles | 90.00, 128.01, 90.00 |
Refinement procedure
| Resolution | 46.100 - 2.600 |
| R-factor | 0.24128 |
| Rwork | 0.238 |
| R-free | 0.29807 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 35942 | |
| Completeness [%] | 96.6 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 40mM K2HPO4, 12% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
