3OQ4
Crystal Structure of motif N of Saccharomyces cerevisiae Dbf4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.714, 99.707, 127.045 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.408 - 2.400 |
R-factor | 0.2049 |
Rwork | 0.204 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oq0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.4_159)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.192 | 0.152 | 0.737 |
Number of reflections | 42318 | ||
<I/σ(I)> | 8.92 | 9.86 | 2.6 |
Completeness [%] | 99.8 | 99.1 | 100 |
Redundancy | 6.2 | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 277 | 22% MPD (v/v) and 100 mM Na/K phosphate buffer pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |