3OQ2

Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	130
Detector technology	CCD
Collection date	2009-11-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	107.874, 48.810, 41.320
Unit cell angles	90.00, 99.20, 90.00

Refinement procedure

Resolution	29.079 - 1.350
R-factor	0.1302
Rwork	0.128
R-free	0.17710
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.350	1.350
Number of reflections	45550
<I/σ(I)>	29.1
Completeness [%]	97.0	97
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Protein solution 0.85 mM DvuCas2, reservoir solution 1 M lithium sulfate, 0.5 M ammonium sulfate and 0.1 M trisodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K