3OQ0

Crystal Structure of motif N of Saccharomyces cerevisiae Dbf4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	90.221, 79.358, 127.419
Unit cell angles	90.00, 110.74, 90.00

Refinement procedure

Resolution	35.520 - 2.700
R-factor	0.2066
Rwork	0.205
R-free	0.24410
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.161
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.4_159))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.100	0.055	0.795
Number of reflections	46033
<I/σ(I)>	16.9	19.31	2.82
Completeness [%]	99.3	98.6	99.1
Redundancy	7	6.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	29% PEG 400, 0.05 M MgCl2, 0.1 M TRIS pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K