3OPV
Crystal structure of E. Coli purine nucleoside phosphorylase Arg24Ala mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | OXFORD RUBY CCD |
Wavelength(s) | 1.54128 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.207, 158.785, 122.240 |
Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
Resolution | 19.100 - 2.400 |
R-factor | 0.182 |
Rwork | 0.181 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k9s |
RMSD bond length | 0.004 |
RMSD bond angle | 0.778 |
Data scaling software | SCALA (CCP4_3.3.9 2008/10/21) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.116 | 19.120 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Total number of observations | 22715 | 49258 | |
Number of reflections | 111867 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 99.8 | 93.38 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 291 | 30% ammonium sulphate, 50mM citric buffer , pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |