3OPU
Crystal structure of the C-terminal domain of Streptococcus mutans surface protein SpaP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9085 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 135.775, 238.391, 78.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.006 - 2.181 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wza |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.500 |
| High resolution limit [Å] | 2.180 |
| Rmerge | 0.049 |
| Number of reflections | 130690 |
| <I/σ(I)> | 28.5 |
| Completeness [%] | 99.7 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 0.1 M sodium acetate pH 5.0, 15% (w/v) polyethylene glycol 6000., VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
