3OPT
Crystal structure of the Rph1 catalytic core with a-ketoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 64 |
Unit cell lengths | 109.415, 109.415, 145.958 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.740 - 2.200 |
R-factor | 0.2003 |
Rwork | 0.198 |
R-free | 0.23560 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.631 |
Data reduction software | HKL-2000 |
Data scaling software | Diffraction (Anisotropy Server) |
Phasing software | PHASES |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.340 |
Number of reflections | 21926 | |
<I/σ(I)> | 21.3 | 1.5 |
Completeness [%] | 77.1 | 39.6 |
Redundancy | 7.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M ammonium sulfate, 0.1M HEPES, 7% PFG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |