3OPQ
Phosphoribosylaminoimidazole carboxylase with fructose-6-phosphate bound to the central channel of the octameric protein structure.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-13 |
Detector | MAR CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.590, 96.670, 128.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.23600 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.477 |
Data scaling software | XSCALE |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.158 | 0.748 |
Number of reflections | 142585 | |
<I/σ(I)> | 10.81 | 2.47 |
Completeness [%] | 99.0 | 100 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 295 | 0.2 M NH4 dihydrogen PO4, 0.1 M Tris, 2% Tacsimate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |