3OPC
Crystal structure of FlgN chaperone from Bordetella pertussis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-19 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97924, 0.9793992, 0.9796705 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.570, 90.390, 49.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.450 - 2.090 |
R-factor | 0.21601 |
Rwork | 0.213 |
R-free | 0.25574 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.199 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 2.100 |
Rmerge | 0.100 | 0.583 |
Number of reflections | 18169 | |
<I/σ(I)> | 19.7 | 2.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.02 M MgCl2, 0.1 M HEPES, 22% polyacrylic acid 5100 sodium salt, strontium chloride 0.01 M, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |