3OP9
Crystal structure of transcriptional regulator from Listeria innocua
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-29 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97967 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 71.120, 71.120, 122.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.098 - 1.898 |
R-factor | 0.1894 |
Rwork | 0.187 |
R-free | 0.22260 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.168 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.053 | 0.591 |
Number of reflections | 12741 | |
<I/σ(I)> | 38.9 | 4 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8.7 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.0 M K/Na tartrate, 0.1 M Tris/HCl, 0.2 M Li2SO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |