3OP8
Crystal structure of the domain V from beta2-glycoprotein I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.290, 38.090, 49.510 |
| Unit cell angles | 93.83, 102.65, 90.09 |
Refinement procedure
| Resolution | 38.000 - 1.900 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.267 |
| Number of reflections | 17881 | |
| <I/σ(I)> | 7.8 | 4.5 |
| Completeness [%] | 94.0 | 93.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1 M (NH4)2SO4, bis-Tris 0.1M, PEG 1500 40 %, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
