3OP7
Crystal structure of a PLP-dependent aminotransferase (ZP_03625122.1) from Streptococcus suis 89-1591 at 1.70 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-23 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.518, 97.518, 89.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.748 - 1.700 |
R-factor | 0.1653 |
Rwork | 0.164 |
R-free | 0.19740 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.671 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.748 | 29.748 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.053 | 0.920 | |
Total number of observations | 3822 | 25552 | |
Number of reflections | 47906 | ||
<I/σ(I)> | 11.2 | 11.7 | 0.8 |
Completeness [%] | 100.0 | 97.1 | 100 |
Redundancy | 7.3 | 6.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.20M lithium sulfate, 2.00M ammonium sulfate, Additive: 0.001M pyridoxal 5'-phosphate (PLP), NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |