3OOY
Crystal structure of human Transketolase (TKT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.230, 117.230, 176.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.480 - 2.050 |
| R-factor | 0.20096 |
| Rwork | 0.199 |
| R-free | 0.23366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.488 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.135 | 0.576 |
| Number of reflections | 85406 | |
| <I/σ(I)> | 6.1 | 2 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 18 % PEG10000, 0.22M Ammonium acetate, 0.1M Bis-Tris pH 5.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
