3OOV
Crystal structure of a methyl-accepting chemotaxis protein, residues 122 to 287
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.132, 60.132, 363.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.520 - 2.200 |
| R-factor | 0.24022 |
| Rwork | 0.237 |
| R-free | 0.30485 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.957 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.520 |
| High resolution limit [Å] | 2.200 |
| Completeness [%] | 99.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 10% w/v PEG 3000, 0.10M phosphate-citrate, pH4.2, 0.20M NaCl. (Wizard II, #36) cryoprotectant: included all of above, in addition to 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
