3OOP
The structure of a protein with unknown function from Listeria innocua Clip11262
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.687, 77.834, 33.065 |
| Unit cell angles | 90.00, 113.36, 90.00 |
Refinement procedure
| Resolution | 47.210 - 1.780 |
| R-factor | 0.21156 |
| Rwork | 0.210 |
| R-free | 0.23638 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.082 | 0.271 |
| Number of reflections | 13923 | |
| <I/σ(I)> | 49.37 | 6.56 |
| Completeness [%] | 96.2 | 81.3 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M calcium acetate, 0.1M tris pH7.0, 20%w/v PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
