3OOP
The structure of a protein with unknown function from Listeria innocua Clip11262
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 64.687, 77.834, 33.065 |
| 格子定数 [度] | 90.00, 113.36, 90.00 |
精密化法
| 分解能 | 47.210 - 1.780 |
| R因子 | 0.21156 |
| Rwork | 0.210 |
| R-free | 0.23638 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.810 |
| 分解能 [Å] (高) | 1.780 | 1.780 |
| Rmerge_l_obs | 0.082 | 0.271 |
| 独立反射数 | 13923 | |
| <I/σ(I)> | 49.37 | 6.56 |
| 完全性 [%] | 96.2 | 81.3 |
| 冗長性 | 4.3 | 3.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M calcium acetate, 0.1M tris pH7.0, 20%w/v PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
