Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.962, 113.244, 258.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.097 - 3.404 |
| R-factor | 0.2636 |
| Rwork | 0.261 |
| R-free | 0.31830 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.883 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.600 |
| High resolution limit [Å] | 3.440 | 6.870 | 3.440 |
| Rmerge | 0.046 | 0.032 | 0.388 |
| Number of reflections | 30339 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 94.6 | 87.5 | 92.2 |
| Redundancy | 1.6 | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15%PEG1000, 360mM Lithium Citrate, 5% Glycerol, 5% Isopropanol, , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
