3OOA
Crystal structures and biochemical characterization of the bacterial solute receptor AcbH reveal an unprecedented exclusive substrate preference for b-D-galactopyranose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.250, 66.117, 177.709 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 33.750 - 2.040 |
R-factor | 0.1704 |
Rwork | 0.168 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | model from SeMet SAD phasing |
RMSD bond length | 0.009 |
RMSD bond angle | 1.047 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.750 | 2.090 | |
High resolution limit [Å] | 2.040 | 9.120 | 2.040 |
Rmerge | 0.104 | 0.026 | 0.538 |
Rmeas | 0.031 | 0.655 | |
Number of reflections | 57305 | 689 | 4119 |
<I/σ(I)> | 9.48 | 25.3 | 2.2 |
Completeness [%] | 98.4 | 97.5 | 96.3 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 2.2M (NH4)2SO4, 100mM citric acid , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |