3OO1
Structure of E. Coli CheY mutant A113P in the absence of Sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.240, 52.952, 37.321 |
Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
Resolution | 19.922 - 1.700 |
R-factor | 0.163 |
Rwork | 0.160 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3chy |
RMSD bond length | 0.005 |
RMSD bond angle | 0.965 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_336)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Number of reflections | 24375 | ||
<I/σ(I)> | 27.1 | 43.9 | 3.3 |
Completeness [%] | 98.9 | 98.4 | 87.4 |
Redundancy | 3.7 | 3.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 28% PEG 8000 100 mM Na Cacodylate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |