3ONX
Crystal structure of a domain of a protein involved in formation of actin cytoskeleton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.307, 67.424, 58.755 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 35.028 - 2.904 |
| R-factor | 0.239 |
| Rwork | 0.235 |
| R-free | 0.27750 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.044 | 0.282 |
| Number of reflections | 17136 | |
| <I/σ(I)> | 15.8 | 2 |
| Completeness [%] | 95.3 | 71.7 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 9% PEG 3350, 85 mM sodium malonate (pH 7.0), 25% glycerol, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
