3ONW
Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-30 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 2 2 21 |
| 格子定数 [Å] | 70.392, 83.679, 190.148 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 95.070 - 2.380 |
| R因子 | 0.22953 |
| Rwork | 0.228 |
| R-free | 0.26539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OM2 chain A |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 28.880 | 2.400 |
| 分解能 [Å] (高) | 2.380 | 2.380 |
| Rmerge_l_obs | 0.065 | 0.761 |
| 独立反射数 | 45328 | |
| <I/σ(I)> | 27.3 | 2.2 |
| 完全性 [%] | 98.8 | 100 |
| 冗長性 | 6 | 6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | Hanging drops were a 1:1 mixture of protein-peptide complex in buffer (10 mM Tris pH 7.5, 1 mM magnesium chloride, 5% (w/v) glycerol, 5 mM DTT) and well solution (1.9 M ammonium sulfate, 100 mM sodium acetate pH 5.0, 200 mM magnesium chloride, 10% (w/v) glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
