3ONV
Crystal structure of E. Coli purine nucleoside phosphorylase with SO4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 120.304, 120.304, 239.809 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.779 - 1.893 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.779 | 47.600 | 2.010 |
| High resolution limit [Å] | 1.890 | 5.650 | 1.890 |
| Rmerge | 0.119 | 0.020 | 1.065 |
| Number of reflections | 81938 | 3449 | 12965 |
| <I/σ(I)> | 23.08 | 87.4 | 2.8 |
| Completeness [%] | 99.9 | 99.1 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 50mM citrate buffer, 28-34 % ammonium sulphate (w/v), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
