3ONO
Crystal Structure of Ribose-5-phosphate Isomerase LacAB_rpiB from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97896 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 62.924, 62.924, 242.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.452 - 1.750 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.304 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 25080 | |
| <I/σ(I)> | 7.1 | 4.9 |
| Completeness [%] | 98.3 | 86.6 |
| Redundancy | 29.1 | 23.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M postassium formate, 20 % (v/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
