3ONN

Crystal structure of 5'-nucleotidase SDT1 from saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	40.720, 77.550, 82.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.775 - 1.870
R-factor	0.2004
Rwork	0.198
R-free	0.24980
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.038
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.720	1.960
High resolution limit [Å]	1.860	1.860
Rmerge	0.071	0.470
Number of reflections	22261
<I/σ(I)>	21.2	4.7
Completeness [%]	98.5	89.8
Redundancy	8.9	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	287	30% PEG 4000, 0.1M Tris-HCl buffer, pH 8.5, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K