3ONM

Effector binding Domain of LysR-Type transcription factor RovM from Y. pseudotuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	69.468, 69.468, 351.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.870 - 2.400
R-factor	0.2243
Rwork	0.221
R-free	0.29460
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.575
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.800	2.530
High resolution limit [Å]	2.400	2.400
Rmerge		0.540
Total number of observations		22745
Number of reflections	17557
<I/σ(I)>	16.4	3.5
Completeness [%]	99.9	100
Redundancy	10.4	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M HEPES pH 8.0, 12.5% (w/v) PEG 6000, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M HEPES pH 8.0, 12.5% (w/v) PEG 6000, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M HEPES pH 8.0, 12.5% (w/v) PEG 6000, vapor diffusion, hanging drop, temperature 298K