3ONJ
Crystal structure of yeast Vti1p_Habc domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.112, 49.751, 51.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.850 - 1.920 |
R-factor | 0.1788 |
Rwork | 0.172 |
R-free | 0.24030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vcs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Number of reflections | 6805 | |
Completeness [%] | 99.8 | 94.7 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 288 | 0.2M Ammonium Sulfate, 0.1M Sodium Acetate trihydrate, 25% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |