3ONJ
Crystal structure of yeast Vti1p_Habc domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.112, 49.751, 51.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.850 - 1.920 |
| R-factor | 0.1788 |
| Rwork | 0.172 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vcs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 6805 | |
| Completeness [%] | 99.8 | 94.7 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 288 | 0.2M Ammonium Sulfate, 0.1M Sodium Acetate trihydrate, 25% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |
