3ONG
Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in Sumo pathways
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.465, 134.038, 62.012 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 38.350 - 2.300 |
| R-factor | 0.22423 |
| Rwork | 0.223 |
| R-free | 0.25099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 22536 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% PEG3350, 0.1 M Bis-Tris pH 5.5, 0.2 M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
