3ON4
Crystal structure of TetR transcriptional regulator from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-29 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97967 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.380, 124.990, 151.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.190 - 1.850 |
R-factor | 0.17947 |
Rwork | 0.179 |
R-free | 0.21781 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.117 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.082 | 0.734 |
Number of reflections | 130984 | |
<I/σ(I)> | 21.5 | 2.4 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M Li2SO4, 0.1 M Tris/HCl, 40% (w/v) PEG400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |