3ON2
Structure of a protein with unknown function from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.889, 86.863, 115.255 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.960 |
| R-factor | 0.17021 |
| Rwork | 0.168 |
| R-free | 0.22157 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.098 | 0.350 |
| Number of reflections | 53066 | |
| <I/σ(I)> | 34.6 | 6.18 |
| Completeness [%] | 99.0 | 94.2 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.2M Li sulfate, 0.1M Bis-Tris pH6.2, 29% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
